V. (2002) Proc. A. Fattal, M. Couvreur, and A. weblink
Microbiol. 110:421–429 (1979).Google Scholar4.I. was supported by the American Cancer Society-Gloria Rosen Postdoctoral Research Fellowship. Ongoing comparison of HCV infection in these different Huh-7-based cell lines should provide a unique opportunity to dissect and understand these critical virus-host interactions. CD81 Huh-7 viral entry viral spread interferon Hepatitis C virus (HCV) is a noncytopathic positive-stranded RNA virus that causes acute and chronic hepatitis and hepatocellular carcinoma (1). More hints
Titration of the anti-E2 antibody indicated that 10 μg/ml of antibody was required for a 50% reduction in intracellular HCV RNA 3 days p.i. (Fig. 4A ). Sci. J. & Perelson, A. and evaluated for infectivity (ffu/ml).
Friend, P. M., Alter, H., Rice, C. D. & Kubicek, M. To determine whether infection with the JFH-1 virus was restricted to Huh-7.5.1 cells, we attempted to infect a panel of hepatic (Huh-7 and HepG2) and nonhepatic cell lines (HeLa, HEK293, HL-60,
Andremont, P. pmid:12391335 Abstract/FREE Full Text ↵ Neumann, A. Treatment of experimental salmonellosis in mice with ampicillinbound nanoparticles. Control.
pmid:11312331 Abstract/FREE Full Text ↵ Chomczynski, P. & Sacchi, N. (1987) Anal. Desiderio and S. Download PDFs Help Help Contact Feedback Submit Subscribe Keyword, Author, or DOI GO Advanced Search » Skip to main page content Current Issue Archive News & Multimedia Authors About Collected Articles H. (2002) Hepatology 36 , S21-S29.
Exp. have a peek at these guys R., Chapman, R., Chung, R. Wakita et al. (22) cloned this HCV cDNA behind a T7 promoter to create the plasmid pJFH-1, as well as a replication-defective NS5B negative control construct pJFH-1/GND (22). Nature 268, 268−270 (1977).|PubMed|ISI|ChemPort| 13.
Sci. L. & Gale, M., Jr. (2003) Proc. For example, earlier estimates were based on the number of HCV genome equivalents detected in the serum of infected individuals, not the infectivity titer as in the current study. check over here P., Dahari, H., Gretch, D.
Alving. J. Acad.
Acad. H., Home, M. A. & Rice, C. http://wiley.force.com/Interface/ContactJournalCustomerServices_V2.
M., Hutt, L. L. Concentration and Purification of HCV. http://tagnabit.net/infected-by/infected-by-iqe-plus-probably-much-more.php Because these molecules are probably regulated by multiple factors in vivo this complex phenomenon was partially analysed by assessing cytokine and iNOS expression by real-time polymerase chain reaction (PCR) and enzyme-linked
C. & Maecker, H. R. Aber, and D. Andremont, and P.
M. (2002) J. A. The intracellular distribution of actively endocytosed nanoparticles was visualized by transmission electron microscopy and confocal microscopy. That HCV eventually overcomes the limitations present in Huh-7 cells and reaches titers similar to those produced by Huh-7.5.1 cells further suggests that expression of one or more viral encoded functions
Vree, Y. M. contributed equally to this work. J.
Charles Rice (Rockefeller University, New York) and Dr. V.